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. Author manuscript; available in PMC: 2009 May 2.
Published in final edited form as: Cell. 2008 May 2;133(3):440–451. doi: 10.1016/j.cell.2008.02.049

Figure 1. Amino acid residues 96-100 in SRP14 are critical for elongation arrest activity of SRP.

Figure 1

(A) Sequence alignment of the C-terminal portions of wild type and mutated human SRP14 proteins (h14).

(B) and (C) Elongation arrest (black) and translocation (gray) activities of SRPs (RCs) reconstituted with different h14 proteins. The right panels: quantification of the results (n≥3). EKRM: 0.2 eq./reaction. Activities were normalized to RCwt (100%.) The elongation arrest and the translocation activities of RCwt were 72±7% and 79±10%, respectively.

(D) Targeting of pPL86-RNCs to microsomes. Targeting efficiencies (T [%]) were monitored by sedimenting microsomes through a sucrose cushion. EKRM: 1 eq./reaction; S: Supernatant, P: Pellet. Right panel: Quantification of the assays (n=3). T [%] was normalized to RCwt (100%).

–RC: Buffer. RC(-14): SRP without h9/14. EKRM: SRP-depleted microsomes.