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. Author manuscript; available in PMC: 2009 May 2.
Published in final edited form as: Cell. 2008 May 2;133(3):440–451. doi: 10.1016/j.cell.2008.02.049

Figure 4. The absence of the elongation arrest function impairs cell growth.

Figure 4

(A) 293T cells were plated at equal densities and live cells counted at the times indicated (n=3). h14 depletion starts at 120 hr (Lakkaraju et al., 2007). No increased cell death was observed with GA12 and GA5 cells (see 0-300 AU in C).

(B) The doubling time was calculated for cell counts between 120-168 hr. Doubling times of 30-34 hr represent a 50-60% increase as compared to 21 hr of the mock treated cells.

(C) Propidium iodide-stained 293T cells were analyzed for cell cycle progression using FACSort. FI: Fluorescence intensity in arbitrary units [AU].

(D) Quantification of cells in different cell cycle phases (n=5). , The sum of cells in the three phases was set to 100%. p<0.05 for cells in G0/G1phase indicates statistical significance of the data at 95% confidence levels.