Fig 5.

Patients’ antibodies react with NR2 subunits of the N-methyl-D-aspartate receptors (NMDARs) expressed by nervous tissue present in the tumor. (A, B) Panels correspond to the ovarian teratoma of Patients 3 and 5 immunolabeled with MAP2 (brown staining), a marker specific for neurons and dendritic processes. Note the intense reactivity with neuronal-like cells and a network of cell processes that are better developed in A. (B, inset) Some immature neuronal cells at higher magnification. (C–E) Panels correspond to the tumor of Patient 3 immunolabeled with the patient’s antibodies (C, green) and a specific antibody for NR2B (E, red). Note that there is colocalization of reactivities (D, yellow), indicating that the patient’s antibodies react with NR2B expressed in the tumor (similar findings were observed with NR2A, not shown). (F–H) Panels correspond to the tumor of Patient 5 immunolabeled with the patient’s antibodies (F, green) and a specific antibody for NR2B (H, red). There is also colocalization of reactivities (G, yellow), indicating that the patient’s antibodies recognize NR2B expressed in the tumor (similar findings were observed with NR2A, not shown). Original magnification ×200, counterstained with hematoxylin (A, B); ×400, immunofluorescence (C–H).