Table 2.
dye | λabsa(nm) | ε a (M−1 cm−1) | λemissa (nm) | Φ a |
---|---|---|---|---|
2b | 548 | 86861 | 554 | 1.00±0.02 c |
2-avidin | 481 | - | 546 | - |
10 | 469 | 17066 | 542 | 0.740±02 d |
11 | 569 | 57032 | 584 | 0.950±02 e |
12 | 477 | 41810 | 584 | 0.70±0.01 d |
11-avidin | 492 | - | 592 | - |
In pH = 7.4 buffer (0.1 N Lithium Phosphate).
In CH2Cl2.
Rhodamine B was used as a standard (Φ = 0.73 in EtOH).
Fluorescein was used as a standard (Φ = 0.92 in 0.1 N NaOHaq).
Rhodamine 101 was used as a standard (Φ = 1.00 in EtOH).