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. Author manuscript; available in PMC: 2008 Jun 18.
Published in final edited form as: Biochem Biophys Res Commun. 2006 Jul 31;348(3):945–949. doi: 10.1016/j.bbrc.2006.07.142

Fig. 1. GST-PKD2WT expression leads to a significant increase in proliferation and invasion of BON cell clones.

Fig. 1

BON cells were stably co-transfected with pcDNA3 (vector), GST-PKD2WT, or GST-PKD2KD by electroporation. A. BON cell clones were lysed and Western blot was performed using anti-PKD2 antibody. B. Following stable transfection, MTT assay was performed. Cells were plated in duplicate; MTT labeling reagent was added, followed by the addition of solubilization solution and measurement of blue formazan production. C. Following stable transfection, Matrigel invasion assay of stable BON cell clones was performed. Cells were plated in triplicate in Matrigel invasion chambers; 5% FBS served as a chemoattractant. Data represent means of triplicate determinations ± SD; * = p<0.05 vs. pcDNA.