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. Author manuscript; available in PMC: 2009 Apr 4.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Jan 22;368(2):273–278. doi: 10.1016/j.bbrc.2008.01.052

Figure 3. Analysis of CG7215 and dPrx5 expression.

Figure 3

A, S2 cells were transfected with in vitro generated dsRNA, complementary to a coding region and part of the 5′UTR of the CG7215 gene. RNA samples were isolated after indicated periods of time followed by RT-PCR analysis with three different sets of primers that amplify the house-keeping rp49 gene (control), as well as the CG7215 and dPrx5 genes. B, RT-PCR analysis of CG7215 and dPrx5 gene expression in the y w strain and a mutant strain, containing a P-element insertion in the coding region of dPrx5. C, immunoblot analysis of dPrx5 protein expression in y w and P-element mutant strains using antibodies developed to dPrx5 recombinant protein. Anti-actin antibodies were used to control for loading.