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. Author manuscript; available in PMC: 2008 Nov 1.
Published in final edited form as: Leukemia. 2007 Oct 18;22(5):1053–1056. doi: 10.1038/sj.leu.2404990

Figure 2. Abi1 pathway is required for Bcr-Abl to induce a polarized distribution of MT1-MMP.

Figure 2

(A) Ba/F3 cells expressing GFP-MT1-MMP were transfected with plain vector (control) or vectors expressing either the wild type Bcr-Abl (p185wt) or mutant Bcr-Abl with deletion of SH3 domain and C-terminal proline-rich sequences (ΔSH3ΔC). The expression of Bcr-Abl proteins was confirmed by Western blotting (Supplementary Figure 2A). GFP-MT1-MMP was visualized by fluorescence microscopy. Arrows indicate polarized distribution of MT1-MMP. Bar: 5 μm. (B) Ba/F3 cells expressing both p185wt and GFP-MT1-MMP were transduced with either control retrovirus (control) or the retrovirus expressing the sequence specific Abi1 shRNA (Abi1 shRNA). The knockdown of Abi1 expression was confirmed by Western blotting as shown in Supplementary Figure 2B. The cells with polarized distribution of GFP-MT1-MMP were visualized by fluorescence microscopy (upper panel) and were counted (lower panel, represented as average percentage of three randomly picked areas). * P<0.01.