Skip to main content
. Author manuscript; available in PMC: 2008 Nov 1.
Published in final edited form as: Leukemia. 2007 Oct 18;22(5):1053–1056. doi: 10.1038/sj.leu.2404990

Figure 3. Knockdown of MT1-MMP expression impaired Bcr-Abl-stimulated cell migration.

Figure 3

(A) Expression of MT1-MMP shRNA resulted in a decrease in mRNA level (lower panel) and proteolytic activity (upper panel) of MT1-MMP in p185wt cells. Ba/F3 cells transformed by p185wt were transduced with control retrovirus (p185wt) or the retrovirus expressing the sequence specific MT1-MMP shRNA (p185wt MMP kd). The proteolytic activity in conditioned medium was analyzed by gelatin zymography and the relative level of MT1-MMP mRNA was analyzed by real time quantitative PCR and the data represents the mean +/- s.d. of triplicate experiments. * P<0.001. (B) Knockdown of MT1-MMP expression impaired the ability of p185wt cells to transmigrate through fibronectin coated membrane. Data represents the mean +/- s.d. of triplicate experiments. * P<0.001.