. Author manuscript; available in PMC: 2008 Jun 18.

Published in final edited form as: J Immunol. 2001 Dec 1;167(11):6356–6365. doi: 10.4049/jimmunol.167.11.6356

Table I.

T cell profile and transduction efficiency of PBMCs post-peptide 209-2M stimulation^a

Transduction on Days Post-Peptide Stimulation				Transduction Efficiency (%)
	T Cell Surface Markers (%)			YFP vector		IL-2YFP vector
	CD3⁺	CD4⁺	CD8⁺	YFP⁺	CD8⁺YFP⁺	YFP⁺	CD8⁺YFP
2	63.47	31.73	52.63	5	4.8	2.35	2
3	65.32	26.1	62.54	10	9.65	3.52	2.88
5	67.5	25.9	64.2	18.61	14.6	6.98	5.47

PBMCs were transduced at the time indicated post-peptide stimulation. Four days post-transduction, equal portions of cells were incubated with PE-conjugated Abs to determine T cell surface markers, the percent of which were determined by FACS (PE channel). Cells were also analyzed for the percent of YFP⁺ and YFP⁺/CD8⁺ to determine the transduction efficiency.