TABLE 2.
Enzymatic kinetics results for selected variants in the pC-XXGRXX library kobs is in the unit of 1/min. R2 and C are defined in the legend to Fig. 4.
| Sequence | kobs × 1000 | R2 | C |
|---|---|---|---|
| WRGRTA | 44.859 | 0.993 | 0.996 |
| LDGRHP | 40.849 | 0.965 | 0.982 |
| QLGRTT | 35.245 | 0.959 | 0.979 |
| PRGRVF | 32.286 | 0.996 | 0.998 |
| SRGRAW | 29.449 | 0.805 | 0.897 |
| QMGRSW | 28.681 | 0.790 | 0.888 |
| FRGRTY | 27.925 | 0.903 | 0.950 |
| LRGRTY | 26.365 | 0.962 | 0.981 |
| GGGRSG | 22.741 | 0.966 | 0.983 |
| lRGRVG | 22.309 | 0.942 | 0.970 |
| MQGRSS | 22.154 | 0.748 | 0.864 |
| LRGRVY | 21.742 | 0.946 | 0.972 |
| LRGRAN | 19.780 | 0.745 | 0.863 |
| LSGRSY | 19.750 | 0.892 | 0.944 |
| ARGRVA | 18.628 | 0.977 | 0.988 |
| YRGRLY | 18.620 | 0.954 | 0.976 |
| YRGRFE | 18.152 | 0.922 | 0.960 |
| CRGRDA | 17.796 | 0.864 | 0.929 |
| LRGRML | 17.119 | 0.972 | 0.985 |
| IQGRMQ | 16.718 | 0.697 | 0.834 |
| PPGRSG | 16.650 | 0.916 | 0.957 |
| YRGRMI | 16.391 | 0.965 | 0.982 |
| VRGRSE | 16.261 | 0.842 | 0.917 |
| TLGRRP | 15.819 | 0.959 | 0.979 |
| LRGRML | 15.741 | 0.985 | 0.992 |
| LQGRMY | 15.645 | 0.953 | 0.976 |
| WQGRNL | 15.107 | 0.705 | 0.839 |
| IQGRLF | 14.332 | 0.919 | 0.958 |
| VSGRMQ | 13.913 | 0.894 | 0.945 |
| QFGRSS | 13.610 | 0.976 | 0.987 |
| SRGRDI | 13.221 | 0.868 | 0.931 |
| PRGRIG | 13.047 | 0.939 | 0.968 |
| SRGRID | 12.469 | 0.770 | 0.877 |
| YWGRFQ | 12.384 | 0.852 | 0.923 |
| QKGRSN | 12.309 | 0.874 | 0.934 |
| SYGRIQ | 12.259 | 0.622 | 0.788 |
| MRGRVN | 11.914 | 0.958 | 0.978 |
| MQGRTL | 11.672 | 0.987 | 0.993 |
| QQGRVL | 11.076 | 0.865 | 0.930 |
| LLGRQY | 10.808 | 0.594 | 0.770 |
| QQGRSE | 10.086 | 0.940 | 0.969 |
| PLGRTQ | 9.914 | 0.926 | 0.962 |
| MQGRAI | 8.689 | 0.795 | 0.891 |
| TLGRFQ | 8.526 | 0.776 | 0.880 |
| IFGRSQ | 8.518 | 0.661 | 0.812 |
| SYGRQQ | 8.469 | 0.981 | 0.990 |
| GQGRWS | 8.259 | 0.656 | 0.809 |
| FRGRLY | 7.917 | 0.852 | 0.922 |
| SQGRSF | 7.883 | 0.611 | 0.781 |
| LEGRQL | 7.833 | 0.647 | 0.804 |
| MQGRNS | 7.410 | 0.952 | 0.975 |
| SQGRTS | 7.263 | 0.836 | 0.914 |
| AQGRSS | 7.211 | 0.620 | 0.787 |
| LVGRSQ | 7.114 | 0.930 | 0.964 |
| YQGRNA | 6.910 | 0.870 | 0.932 |
| YFGRTQ | 6.723 | 0.848 | 0.921 |
| QLGRGA | 6.718 | 0.919 | 0.958 |
| QLGRTT | 6.346 | 0.954 | 0.976 |
| MQGRNS | 6.321 | 0.842 | 0.917 |
| TRGRFL | 6.290 | 0.943 | 0.971 |
| LIGRMQ | 6.251 | 0.519 | 0.720 |
| CRGRYF | 5.792 | 0.746 | 0.863 |
| NLGRQS | 5.419 | 0.758 | 0.870 |
| SNGRSS | 5.361 | 0.745 | 0.863 |
| QSGRFH | 5.020 | 0.141 | 0.375 |
| QYGRYN | 4.592 | 0.839 | 0.916 |
| NVGRVQ | 3.797 | 0.113 | 0.335 |
| LCGRQL | 3.721 | 0.414 | 0.643 |
| LQGRIS | 3.514 | 0.702 | 0.837 |
| TQGRSS | 2.996 | 0.638 | 0.798 |
| DLGRWD | 2.919 | 0.115 | 0.338 |
| SFGRAQ | 2.217 | 0.200 | 0.446 |
| SVGRAQ | 1.422 | 0.664 | 0.815 |
| VRGRFW | 0.824 | 0.644 | 0.802 |
| QNGRLF | 0.817 | 0.149 | 0.386 |