Three possible topologies illustrating the relationships among neurohypophysial hormone precursors of Australian lungfish, toad, mammals, and teleosts. The lamprey VT precursor was used as outgroup. Tree A, monophyly of the lungfish and the toad; tree B, monophyly of the lungfish and tetrapods; tree C, monophyly of the lungfish and teleosts. The most likely tree among the topologies analyzed by the maximum likelihood method, as shown in Table 1, is tree A.