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. 2008 May 2;283(18):12026–12033. doi: 10.1074/jbc.M704592200

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Copyright © 2008, The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — The monomer to dimer ratio varies between different DDR1 isoforms. MCF7 cells stably transfected with DDR1a-FLAG and DDR1d-FLAG were stimulated with collagen for 18 h and lysed. DDR1 expression was detected by the monoclonal anti-FLAG antibody (1:1000 dilution). A, DDR1a was detected as a monomer (125 kDa, arrow) under reducing conditions and as both a monomer and dimer (250 kDa, chevron) under nonreducing conditions irrespective of collagen (Col) stimulation. B, DDR1d was detected as a monomer (75 kDa, arrow) under reducing conditions and as both a monomer and dimer (150 kDa, chevron) under nonreducing conditions irrespective of collagen stimulation. These experiments were carried out a minimum of three times and very similar results obtained.