FIGURE 7.

Morphogenesis and patterning in RNAiB2 larvae. A, d-TFB2M silencing does not affect muscular tissue morphogenesis. A transgenic UAS-IRB2 line that expressed the reporter gene LacZ under control of the myosin enhancer was generated to evaluate the morphology of the muscles in RNAiB2 larvae. Reporter expression is revealed by β-galactosidase staining. B, TFB2M silencing does not affect nervous system morphogenesis. Larval central nervous system and neuromuscular joints were visualized by immunostaining with an antibody against horseradish peroxidase (red). Images from neuromuscular joints between muscle fibers 6–7 and 13–14 from the third abdominal segment were obtained by confocal fluorescence microscopy. C, d-TFB2M silencing does not affect the expression pattern of wingless. The expression of the wingless gene was visualized by immunostaining (red) in wing imaginal discs of RNAiB2 larvae and control larvae.