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. 2008 May 2;283(18):12305–12313. doi: 10.1074/jbc.M801291200

FIGURE 1.

FIGURE 1.

Effect of adenoviral infection, siRNA, or metabolic stress on Hsp27 expression and cell survival after metabolic stress. A, Hsp27 level in lysates harvested from non-infected cells (Ctl), cells subjected to transient heat stress (HS), or infected with adenovirus containing either empty vector (EV) or human Hsp27 (Hsp27). Lower panel, β-tubulin loading control. B, Hsp27 content after exposure to non-sense siRNA (NS siRNA) or specific Hsp27 siRNA (Hsp27 siRNA). Lower panel, β-tubulin loading control. C, Hsp27 content at base line (Base), after 60 min metabolic stress (ATP Depl), and after 30 min of recovery (Rec30). D, cell survival after 1 h of metabolic stress followed by 30 min of recovery in empty vector (EV), Hsp27 adenovirus (Hsp27 AdV), non-sense siRNA (NS siRNA), and Hsp27 siRNA (Hsp27 siRNA)-treated cells compared with base line. Data are the mean ± S.E. and were performed in triplicate; *, p < 0.05 versus base-line survival; immunoblots are representative of at least three independent experiments.