Effect of adenoviral infection, siRNA, or metabolic stress on Hsp27
expression and cell survival after metabolic stress. A, Hsp27
level in lysates harvested from non-infected cells (Ctl), cells
subjected to transient heat stress (HS), or infected with adenovirus
containing either empty vector (EV) or human Hsp27 (Hsp27).
Lower panel, β-tubulin loading control. B, Hsp27
content after exposure to non-sense siRNA (NS siRNA) or specific
Hsp27 siRNA (Hsp27 siRNA). Lower panel, β-tubulin
loading control. C, Hsp27 content at base line (Base), after
60 min metabolic stress (ATP Depl), and after 30 min of recovery
(Rec30). D, cell survival after 1 h of metabolic
stress followed by 30 min of recovery in empty vector (EV), Hsp27
adenovirus (Hsp27 AdV), non-sense siRNA (NS siRNA), and
Hsp27 siRNA (Hsp27 siRNA)-treated cells compared with base line. Data
are the mean ± S.E. and were performed in triplicate; *,
p < 0.05 versus base-line survival; immunoblots are
representative of at least three independent experiments.