TABLE 1.
Spectroscopic and photochemical properties of DrBphP variants
+ or — indicates expression or solubility are good or poor, respectively. NA indicates not applicable and ND indicates not determined.
| Construction | Expression/solubility | BV covalent attachment | Pr λmax | Red-irradiated λmax | Q/Soret ratio | Photoconversion | Porphyrin fluorescence |
|---|---|---|---|---|---|---|---|
| nm | nm | ||||||
| Wild type | +/+ | Yes | 700 | 751 | 2.69 | Pr/Pfr | No |
| ΔN1-20 | +/+ | Yes | 701 | 752 | 2.34 | Intermediate | ND |
| E25A/E27A | +/+ | Yes | 698 | 751 | 2.23 | Pr/Pfr | No |
| 135A | +/+ | Yes | 699 | 751 | 2.34 | Intermediate | ND |
| Q36A | +/- | Yes | NA | NA | NA | NA | NA |
| Q36D | +/+ | Yes | 698 | 752 | 2.47 | Pr/Pfr | ND |
| Q36K | +/- | Yes | NA | NA | NA | NA | NA |
| Q36L | +/+ | Yes | 701 | 750 | 2.62 | Pr/Pfr | ND |
| Q36N | +/- | NA | NA | NA | NA | NA | NA |
| P37A | +/+ | Yes | 700 | 756 | 2.42 | Pr/Pfr | ND |
| Y176H | +/+ | Yes | 696 | 750 | 1.28 | Intermediate | No |
| F203A | +/+ | Yes | 696 | 738 | 2.13 | Intermediate | No |
| F203H | +/+ | Yes | 695 | 742 | 2.16 | Intermediate | No |
| F203W | +/+ | Yes | 701 | 751 | 2.37 | Pr/Pfr | No |
| D207A | +/+ | Yes | 700 | 751 | 1.91 | Intermediate | Yes |
| D207E | +/+ | Yes | 701 | 751 | 2.20 | Intermediate | No |
| D207H | +/+ | Yes | 700 | NA | 0.83 | Locked in Pr | Yes |
| D207K | +/+ | Yes | 700 | NA | 1.95 | Locked in Pr | Yes |
| D207L | +/+ | Yes | 700 | NA | 1.93 | Locked in Pr | Yes |
| D207N | +/+ | Yes | 700 | 751 | 1.74 | Intermediate | Yes |
| D207Q | +/+ | Yes | 701 | 746 | 2.02 | Intermediate | Yes |
| D207S | +/+ | Yes | 701 | NA | 1.59 | Locked in Pr | Yes |
| D207T | +/+ | Yes | 704 | 751 | 1.87 | Intermediate | Yes |
| I208A | +/+ | Yes | 695 | 751 | 2.26 | Pr/Pfr | No |
| P209G | +/+ | Yes | 690 | 745 | 1.97 | Pr/Pfr | ND |
| Y216H | +/+ | Yes | 697 | 748 | 2.42 | Pr/Pfr | No |
| Y216W | +/+ | Yes | 698 | 751 | 0.67 | Intermediate | ND |
| R254A | +/+ | Yes | 700 | 750 | 1.14 | Pr/Pfr | ND |
| R254K | +/+ | Yes | 701 | 750 | 2.45 | Pr/Pfr | ND |
| R254Q | +/+ | No | NA | NA | NA | NA | NA |
| H260A | +/+ | Yes | 698 | 748 | 1.16 | Intermediate | No |
| H260D | +/+ | No | NA | NA | NA | NA | NA |
| H260K | +/+ | No | NA | NA | NA | NA | NA |
| H260N | +/+ | Yes | 698 | 754 | 2.62 | Intermediate | No |
| H260Q | +/+ | Yes | 697 | 748 | 2.35 | Pr/Pfr | No |
| H260S | +/+ | Yes | 694 | 748 | 1.50 | Intermediate | ND |
| Y263H | +/+ | Yes | 698 | 742 | 1.78 | Intermediate | Yes |
| H290N | +/+ | Yes | 699 | 747 | 2.95 | Intermediate | No |
| H290Q | +/+ | Yes | 702 | 746 | 2.58 | Intermediate | Yes |