FIGURE 8.
Effect of CHP3 on biosynthetic maturation of NHE1. AP-1 cells were transiently cotransfected with NHE1HA and empty vector or CHP3myc. Cell lysates were prepared at the indicated time points following transfection and subjected to SDS-PAGE and immunoblotting to detect expression of fully glycosylated (fg) and core-glycosylated (cg) forms of NHE1HA and CHP3myc as described in the legend to Fig. 6. Blots were stripped and reprobed for expression of endogenous GAPDH as a control for protein loading. Data shown are representative of three independent experiments.