FIGURE 4.
PTEN negatively regulates Akt in response to ONOO-.
A, PTEN activity in HUVECs exposed to ONOO- (n =
5; 
, p < 0.01
compared with control). B, PTEN null MDA-MA-468 cells were exposed to
varying concentrations of ONOO- for 15 min. Phosphorylation of
PKCζ, LKB1, and Akt was analyzed by Western blot. C, MDA-MA-468
cells were transiently transfected with phosphatase-dead mutant PTEN (C124S)
or LacZ prior to ONOO- exposure (5 μm, 15 min).
Lysates were analyzed for phosphorylated Akt-Ser473, Akt, and PTEN.
Representative blots are shown from three separate experiments.