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. 2008 May 2;283(18):12267–12275. doi: 10.1074/jbc.M708523200

FIGURE 2.

FIGURE 2.

Analysis of OXPHOS complex levels by BN-PAGE. A, mitochondrial extracts isolated from spinal cords of 3–4-week-old NTG, G93A SOD1, CCS/WT SOD1, and CCS/G93A SOD1 mice fractionated by BN-PAGE and probed with anti-39-kDa (complex I), anti-Fp70 (complex II), anti-core-1 (complex III), anti-COX1 (complex IV)- and anti-ATPase α (complex V) antibodies, 10 μg of protein/lane. Each lane represents a unique animal. B, quantification of complex levels on blue native gel. CCS/G93A SOD1 show significant a decrease in levels of complex IV. #, p < 0.04 for CCS/G93A SOD1 versus NTG by ANOVA followed by Tukey's post-hoc test. CCS/G93A SOD1 mice show significant increases in complex I, II, and III levels. p < 0.004 (*) and p < 0.001 (**) for CCS/G93A SOD1 versus NTG. n = 3–5 animals for each genotype. Levels of complexes I, II, III, IV, and V are normalized to the median NTG value.