FIGURE 5.

Analysis of complex IV assembly in CCS/G93A SOD1 spinal cord. Mitochondrial extracts from the spinal cords of 3–4-week-old CCS/G93A SOD1 mice (A and C) and NTG controls (B and D) were analyzed by BN-PAGE and two-dimensional SDS-PAGE immunoblots. Blots were probed with antibodies against COX1, COX2, and COX4. C and D are lighter exposures of A and B, respectively. The asterisk indicates S1 subcomplex containing COX1; 20 μg of protein was loaded per lane in BN-PAGE. Experiments were performed in duplicate with two sets of animals. A representative experiment is shown.