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. 2008 Apr 4;283(14):9089–9100. doi: 10.1074/jbc.M710012200

FIGURE 2.

FIGURE 2.

Mitochondrial targeting of WT/α-synuclein and +33/α-synuclein in DAN neuronal cultures. DAN cells were transfected with WT/synuclein-FLAG (A) and +33/synuclein-FLAG (D). Cytosol (cyto.) and mitochondrial (mito.) fractions (100 μg) were probed with FLAG antibodies. B and C show the levels of loading controls: TOM20 and actin, respectively, for A. E and F show the levels of loading controls: TOM20 and actin, respectively, for D. G, quantitation of WT/synuclein and +33/synuclein in the mitochondrial and cytosolic fractions by ELISA at different time points of transfection. Values are mean ± S.D. from three separate experiments. *, p < 0.05 compared with 20-h transfection. H, following the transfection, the whole cell lysates (homogenate) from DAN cells were tested for the levels of α-synuclein at different time points using quantitative ELISA. Values are mean ± S.D. from three separate experiments. No significant variation was observed (one-way analysis of variance with Tukey-Kramer post test).