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Mitochondrial localization of WT/α-synuclein and +33/α-synuclein by immunofluorescence microscopy. DAN cells were transfected with WT/synuclein-FLAG (A–C) or +33/synuclein-FLAG (D–F). Cells (A and B, and D and E) were double immunostained with antibodies to FLAG and TOM20, respectively. C and F are the merged image of A and B, and D and E, respectively. Bar = 10 mm.
