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. 2008 Apr 4;283(14):9089–9100. doi: 10.1074/jbc.M710012200

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FIGURE 4. — Mitochondrial complex activities in DAN cells expressing various α-synuclein constructs. A, Western immunoblot analysis of mitochondrial (100 μg) localization of WT/synuclein-FLAG and A53T/synuclein-FLAG with time using antibodies to FLAG. B, levels of TOM20. Mitochondrial complex I activity (C) and the production of ROS (D) were estimated at various time points following the expression of WT/synuclein-FLAG, A53T/α-synuclein-FLAG, and +33/synuclein-FLAG cDNA constructs. ^*, p < 0.05 compared with vector control. ^**, p < 0.05 compared with vector control, and +33/α-synuclein-FLAG constructs. ^#, p < 0.05 compared with WT/synuclein-FLAG (one-way analysis of variance with Tukey-Kramer post test). Western immunoblot analysis of endogenous α-synuclein (E), actin (F), and TOM20 (G) in total homogenate (100 μg) and mitochondria (100 μg) following 48-h transfection of α-synuclein-specific siRNA in DAN cells. Mitochondrial NADH cytochrome c reductase (H) and complex I (I) in control and α-synuclein-specific siRNA transfected DAN cells. ^*, p < 0.05 (Student t test).