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. 2008 Apr 4;283(14):9089–9100. doi: 10.1074/jbc.M710012200

FIGURE 6.

FIGURE 6.

Biochemical and immunoelectron microscopy characterization of mitochondrial α-synuclein in human PD and normal brains. Western blot analysis of 50 μg of mitochondrial fraction from substantia nigra of PD #1 for α-synuclein (A), mitochondrial (mt)HSP70 (B), CcO (C), and TOM20 (D) following the treatment with trypsin, digitonin, and sodium carbonate. Sup = supernatant, Dig = digitonin. E, Western blot analysis of 50 μg of cytosol from substantia nigra of PD #1 treated with or without trypsin (30 μg/mg of protein) using antibodies to α-synuclein. Immunoelectron microscopy analysis of α-synuclein in the mitochondria of substantia nigra of PD subject (PDS #1) (F) and normal subject (NS #11) (G) using anti rabbit antibodies toα-synuclein. The sections were stained with secondary antibody conjugated to 20 nm (E) and 10 nm (F), respectively. M = mitochondrion, V = vesicle, C = cytosol. Bar = 100 nm. Arrows indicate association of gold particle to the outer membrane of mitochondria.