FIGURE 3.

Global gene expression patterns in control and Brd4 KD NIH3T3 cells. A, coefficient of correlation (R²) was calculated based on scatter plot analysis of microarray results. B, biplot scores for control and Brd4 KD cells at 0, 4, and 8 h were obtained according to the first two components of the principal component analysis. C, the number of genes whose expression was altered by Brd4 shRNA. ^*, the number of up-regulated genes listed was most likely an overestimate, since in qRT-PCR only 2 of 20 genes in this category showed higher expression in Brd4 KD cells compared with control cells. This discrepancy may be due to a normalization procedure used in this work.