FIGURE 5.

Cell cycle-dependent recruitment of Brd4 to G₁ gene promoters. A, ChIP analysis was performed with control or Brd4 KD cells synchronized to G₀ (0) and released for 4 h (4). Chromatin was precipitated with rabbit antibody for Brd4 and Cdk9 or for RNA pol II (N20) along with rabbit IgG as a control (IgG). Precipitated chromatin was analyzed for the indicated genes by quantitative PCR by detecting a region containing/near transcription initiation site. Values represent the average of three determinations ± S.D. Similar results were observed with three independent chromatin preparations. B, immunoblot analysis was performed for total extracts prepared from control and Brd4 KD cells synchronized to G₀ and released at 0 h and 4 h.