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Parameters derived from Perrin plots of MIANS-labeled GFP fusion proteins MIANS was attached to the C-terminal cysteine residue.
| Protein | y intercept (1/Ao) | Slope (centipoises/K × 103) | τc |
|---|---|---|---|
| ns | |||
| GFP-CTD5 | 3.9 ± 0.3 | 7.4 ± 0.1 | 5.1 ± 0.6 |
| GFP-CcdA41-CTD5 | 3.9 ± 0 | 31.6 ± 0.9 | 2.2 ± 0.1 |
| GFP-CcdA41-CTD5 K193S | 4.6 ± 0.1 | 31 ± 1 | 2.8 ± 0.2 |
| GFP-CcdA41-CTD5 V196S | 4.4 ± 0.4 | 24 ± 1 | 2.9 ± 0.5 |
| GFP-CcdA41-CTD5 V196C | 4.2 ± 0.2 | 29 ± 1 | 2.2 ± 0.1 |
