FIGURE 5.

Inhibition of histone deacetylase activity by TSA up-regulates neuronal gene transcription in HNSC. 100 human neural stem cells. A, HNSC.100 cells were treated for 24 h with the histone deacetylase inhibitor TSA (25 ng/ml) or with the vehicle Me₂SO. RNA from Me₂SO-treated (denoted -) and TSA-treated (denoted +) cells was isolated and analyzed by RT-PCR. The expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as a control. B, quantification and statistical analysis of the data shown in A.