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. 2008 Apr 4;283(14):9231–9238. doi: 10.1074/jbc.M708077200

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Copyright © 2008, The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Rescue of IOP1 knockdown effect by siRNA-resistant IOP1. HeLa cells stably transfected with pcDNA3 or pcDNA3-HA-IOP1^R were transfected with the indicated siRNAs. A, total cellular extracts (30 μg) were analyzed by Western blotting using antibodies against IOP1 (upper panel), the HA tag (middle panel), or β-tubulin (lower panel). The positions of endogenous IOP1 and the stably expressed HA-IOP1^R are as indicated. B-D, cytosolic or mitochondrial extracts were assayed for aconitase activity as indicated. Shown are the means ± S.D., n = 3. ^*, p < 0.01; ^**, p < 0.05.