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. 2008 Apr 4;283(14):9341–9349. doi: 10.1074/jbc.M707807200

FIGURE 2.

FIGURE 2.

Fragmentation and chromatography of endogenous GP-NAEs in mouse brain via LC-MS/MS. Fragmentation and chromatographic behavior are shown for endogenous N-palmitoyl (A-C) and N-oleoyl (D-F) GP-NAEs. Three major fragment ions are produced, a diagnostic NAE-phosphate fragment (378 and 404 for palmitoyl- and oleoyl-GP-NAEs, respectively) and two fragments corresponding to glycerol 3-phosphate and its dehydrate at 171 and 153, respectively (A and D). B and E, representative multiple reaction monitoring chromatographs of synthetic and endogenous GP-NAEs. C and F, tandem MS/MS fragmentation spectra of synthetic and endogenous GP-NAEs.