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GDE1 and GP-NAE phosphodiesterase activity reside in the membrane. A, Western blot of mouse brain GDE1 showing that it resides exclusively in the membrane fraction and is a glycoprotein as evidenced by a ∼5-kDa shift in migration upon treatment with PNGase F. B, GP-NAE phosphodiesterase activity resides exclusively in the membrane fraction of mouse brain.
