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N-Acyl chain selectivity of GDE1. Membrane fractions from GDE1-transfected COS-7 cells were tested for activity against a panel of GP-NAE substrates bearing saturated (C16:0, C20:0), monounsaturated (C18:1), and polyunsaturated (C20:4, C22:6) N-acyl chains. Assays were performed with 100 μm of each substrate using LC-MS to measure release of NAE.
