Effect of dNTP concentration on DNA synthesis by the HIV-1 RTs. A
DNA primer labeled at the 5′-end was annealed to a 520-nt RNA template,
and extension assays were performed with excess wild-type, M184I, and M184A
RTs at dNTP concentrations of 5, 50, and 100 μm each. The
reactions were stopped at 15, 30, and 60 s. L, 25-bp DNA ladder.