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. 2008 Apr 4;283(14):9196–9205. doi: 10.1074/jbc.M710148200

FIGURE 3.

FIGURE 3.

Effect of dNTP concentration on DNA synthesis by the HIV-1 RTs. A DNA primer labeled at the 5′-end was annealed to a 520-nt RNA template, and extension assays were performed with excess wild-type, M184I, and M184A RTs at dNTP concentrations of 5, 50, and 100 μm each. The reactions were stopped at 15, 30, and 60 s. L, 25-bp DNA ladder.