FIGURE 1.

Schematic representation of SgII domain GFP, EAP, or CFP chimeric proteins designed to study SgII trafficking to DCGs in sympathoadrenal cells. Sequences corresponding to full-length human SgII or indicated fragment were fused in-frame to GFP, EAP, or GalT-CFP reporters, under the control of the cytomegalovirus promoter. Plasmid numbering refers to the position of the SgII fragment subcloned, within the original human SgII cDNA (NCBI NM_003469). For instance, in pCMV-SgII1084-EGFP (or EAP), the 3′ end of the subcloned fragment (at amino acid 301) is located at 1084 bp in the original cDNA. Numbers along the diagram of the chimeric proteins indicate amino acid residues positions. Proteins are drawn proportional to scale.