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CDK2 activity is required for the cooperating effects of cyclin E and st in density-arrested NHF. BJ-hTERT fibroblasts were grown to high density and transduced with the indicated adenoviruses. Four hours later, roscovitine (25 μm) or vehicle (Me2SO (DMSO)) was added to the medium. Cells were collected at the indicated time points and processed for PI/FACS (A) or Western blot (B) analyses.
