FIGURE 7.

Coexpression of st and cyclin E forces exit from quiescence induced by density arrest or CKI overexpression. BJ-hTERT fibroblasts were grown to high density and then transduced with the indicated recombinant adenoviruses and harvested at the indicated time points for PI/FACS (A) and Western blot (B) analyses. Exponentially growing BJ-hTERT fibroblasts were transduced with recombinant adenoviruses expressing p21, p27, or control adenoviruses. Eight hours later, BJ-hTERT fibroblasts were transduced with adenoviruses expressing cyclin E. After 48 h, cells were transduced with adenoviruses expressing st. BJ-hTERT fibroblasts were harvested at the indicated time points following transduction with st adenoviruses and analyzed by PI/FACS (C) and by Western blot (D) analyses.