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The FRT sequence. A, the full FRT site, with arrows marking the 3 Flp-binding subsites. Positions discussed in the text are numbered. B, the WT FRT suicide substrate used in binding and cleavage assays (variations are shown in Table 1). Vertical arrow marks the cleavage site, and several Flp side chains are shown next to the bases they are in close proximity to (see Fig. 1).
