TABLE 2.

Flp-catalyzed DNA cleavage rates for 13 FRT variants

	Ki	Fraction bound	vo	kcleave
	nm		s–1 × 10–3
WT	4.7 ± 1.3	0.95 ± 0.012	2.7 ± 0.34	2.8 ± 0.06
Frt1ta	0.96 ± 0.02	0.99 ± 0.0002	3.1 ± 0.15	3.1 ± 0.1
Frt1gc	180 ± 2.2	0.36 ± 0.003	0.0108 ± 0.0047	0.03 ± 0.04
Frt14ta	17 ± 3.4	0.85 ± 0.025	0.29 ± 0.072	0.3 ± 0.01
Frt46ta	212 ± 59	0.32 ± 0.06	0.043 ± 0.0033	0.1 ± 0.03
Frt4ta	15 ± 2.2	0.87 ± 0.017	0.54 ± 0.055	0.6 ± 0.04
Frt6ta	20 ± 2.1	0.83 ± 0.015	0.87 ± 0.014	1.0 ± 0.02
Frt3at	228 ± 26	0.30 ± 0.024	0.1 ± 0.017	0.33 ± 0.03
Frt2at	2.6 ± 1.1	0.97 ± 0.01	4.9 ± 0.28	5.0 ± 0.05
Frt5cg	106 ± 30.2	0.48 ± 0.07	0.9 ± 0.11	1.9 ± 0.27
Frt4cg	49 ± 5.6	0.67 ± 0.025	0.64 ± 0.032	0.96 ± 0.04
Frt3cg	137 ± 0.33	0.42 ± 0.0006	0.5 ± 0.046	1.2 ± 0.02
Frt3au	43 ± 6.8	0.70 ± 0.03	0.9 ± 0.025	1.3 ± 0.07