Figure 6.

Southern analysis of the TSA gene structure in bxbx and FRB73 maize lines. In the Southern blot lanes 1–4 contain 5 μg of SphI- or EcoRI/SphI-digested genomic DNA from bxbx or FRB73 maize seedlings. The blot was probed with the EcoRI fragment from the TSA genomic clone (as shown in the schematic). The cartoon is a schematic of the TSA gene structure and the location of the proposed deletion in the bxbx maize line. The open boxes indicate the TSA exons. The restriction sites shown are EcoRI (E) and SphI (S); the restriction sites in bold are known from the sequence of the TSA genomic clone, and the sites in parentheses are inferred from the Southern data above.