. 2008 Jul;49(7):1477–1487. doi: 10.1194/jlr.M800030-JLR200

TABLE 1.

Phospholipids increased by knock-down of iPLA₂γ in RPTCs

Positive mode phospholipids	[M+H]
	865
Control	−0.52 ± 0.41^a
shRNA, 24 h	1.26 ± 0.38^b
shRNA, 48 h	−0.17 ± 0.52^a
Scrambled, 24 h	−0.17 ± 0.24^a
Scrambled, 48 h	−0.55 ± 0.13^a
Negative mode phospholipids	[M-H]
	578	693	725	730	795	844	860	873	985
Control	−0.27 ± 0.13^a	−0.37 ± 0.07^a	−0.23 ± 0.13^a	−0.31 ± 0.07^a	−0.36 ± 0.07^a	−0.23 ± 0.14^a	−0.29 ± 0.19^a	−0.38 ± 0.03^a	−0.21 ± 0.06^a
shRNA, 24 h	1.31 ± 1.01^b	−0.35 ± 0.13^a	−0.25 ± 0.13^a	1.18 ± 0.92^b	1.16 ± 0.94^b	−0.30 ± 0.16^a	−0.49 ± 0.32^a	0.09 ± 0.29^ab	1.32 ± 1.09^b
shRNA, 48 h	−0.33 ± 0.07^a	1.27 ± 0.75^b	1.18 ± 0.81^b	−0.27 ± 0.15^a	−0.38 ± 0.12^a	1.15 ± 0.80^b	1.21 ± 0.61^b	1.05 ± 0.84^b	−0.32 ± 0.00^a
Scrambled, 24 h	−0.40 ± 0.00^a	−0.31 ± 0.20^a	−0.39 ± 0.12^a	−0.46 ± 0.08^a	−0.46 ± 0.11^a	−0.24 ± 0.19^a	−0.02 ± 0.23^a	−0.39 ± 0.07^a	−0.20 ± 0.11^a
Scrambled, 48 h	−0.05 ± 0.30^a	−0.36 ± 0.07^a	−0.53 ± 0.03^a	−0.45 ± 0.13^a	−0.50 ± 0.35^a	−0.35 ± 0.10^a	−0.32 ± 0.00^a

iPLA₂, Ca²⁺-independent phospholipase A₂; [M+H], gain of a proton; [M−H], loss of a proton; small hairpin ribonucleic acid, shRNA; RPTC, renal proximal tubular cell. iPLA₂γ was knocked down in RPTCs and whole cell lipids were isolated using a modified Bligh and Dyer (19) method. Phospholipids were identified using ESI-MS in both positive and negative ion modes. Data were normalized using the central limit theorem as described in Experimental Procedures and are presented as means ± SEM from four or five different rabbits. Within lipid species, means with the same letters were not significantly different as determined by Duncan's multiple range test. Where SEM is equal to zero, the particular lipid species was not identified by ESI-MS in any of the samples from that treatment. Boldface values are statistically significant within that particular phospholipid.