Quantitative real-time PCR of StarD4, StarD1, and StarD5 mRNA in primary mouse hepatocytes. Following a 2 h transfection with Ad-CMV StarD4, StarD1, or StarD5, levels of the mRNAs were increased in a time-dependent manner over control Ad-CMV transfected hepatocytes. Data are presented as percentage increase over controls (means ± SEM of three independent experiments).