Fig. 4.

Effects of overexpressed luciferase and Elovl-5 on hepatic gene expression in fasted and fed C57BL/6 mice. Total RNA was extracted from livers of fasted and refed mice infected with either Ad-Luc or Ad-Elovl5. Transcript abundance was measured by quantitative real-time PCR (Table 1) (17). Transcripts measured included cyclophilin, L-PK (A), SREBP-1 (B), FAS (C), stearoyl-CoA desaturase (SCD1; D), CYP4A10 (E), phosphoenolpyruvate carboxykinase (PepCk; F), HMG-CoA synthase (G), and ABCA1 (H). Results are expressed as relative transcript abundance (transcript/cyclophilin; mean ± SD, n = 4). For panels A–C, * P < 0.05, by Student's t-test; for panels D–G, P values are shown, by ANOVA.