Fig. 5.

Effects of overexpressed luciferase or Elovl-5 on the nuclear abundance of hepatic nuclear factor-4α (HNF-4α), SREBP-1, carbohydrate-regulatory element binding protein (ChREBP), Max-like factor X (MLX), PPARα, and TATA binding protein (TBP). Hepatic nuclear proteins were prepared from fasted and fed Ad-Luc- and Ad-Elovl5-infected mice, electrophoretically separated, and assayed for HNF-4α, SREBP-1, ChREBP, MLX, PPARα, and TATA binding protein by immunoblotting (upper panel). Immunoblots were quantified using LiCor Odyssey. These results are normalized to the protein abundance in fasted Ad-Luc-infected animals. Results are presented as fold change in nuclear protein abundance (mean ± SD, n = 3). The results reported here are representative of two or more separate experiments. * P < 0.01, Ad-Luc versus Ad-Elovl5, by ANOVA.