TABLE 1.
Primer pairs for quantitative real-time PCR
| Enzyme | Accession Number | Forward | Reverse |
|---|---|---|---|
| Mouse | |||
| Acetyl-CoA carboxylase-1 | NM_133360 | TGTCCGCACTGACTGTAACC | ATTTCCATAGCCGACTTCCA |
| Acyl-CoA oxidase | NM_015729 | CCCAACTGTGACTTCCATCA | ACGGATAGGGACAACAAAGG |
| ATP binding cassette A1 | NM_013454 | TCATGGAGAACAGCCAAGAG | GGGCAGTCCAATCTAATCCA |
| Cyclophilin | NM_008907.1 | CTTCTTGCTGGTCTTGCCATTCCT | TGGATGGCAAGCATGTGGTCTTTG |
| Cytochrome P450 4A10 | NM_010011 | TGTTTGACCCTTCCAGGTTT | CAATCACCTTCAGCTCACTCA |
| Cytochrome P450 7A | NM_007824 | GGGATTGCTGTGGTAGTGAG | AGCCCAGGTATGGATCAAC |
| Fatty acid synthase | NM_007988 | CAAATACAATGGCACCCTGA | TGGCGAAGCCGTAGTTAGTT |
| Glucokinase | NM_010292 | GAGATGGATGTGGTGGCAAT | ACCAGCTCCACATTCTGCAT |
| Glucose transporter-2 | NM_031197 | GAGGAAGTCAGGGCAAAGAA | AAGAGCTGGATCACGGAGAC |
| Interleukin 6 | NM_031168 | AGACAAAGCCAGAGTCCTTCA | AGGAGAGCATTGGAAATTGG |
| L-pyruvate kinase | NM_013631 | AGGAGTCTTCCCCTTGCTCT | ACCTGTCACCACAATCACCA |
| HMG-CoA synthase-2 | NM_008256 | CCTTGAACGAGTGGATGAGA | CAGATGCTGTTTGGGTAGCA |
| Phosphoenolpyruvate carboxykinase | NM_011044 | ACATTGCCTGGATGAAGTTTG | GGCATTTGGATTTGTCTTCAC |
| Sterol-regulatory element binding protein-1 | NM_011480 | AGGAGAACCTGACCCTACGA | GGTAAGCGTCTCCACCACTT |
| Stearoyl-CoA desaturase-1 | NM_009127 | TCAACTTCACCACGTTCTTCA | CTCCCGTCTCCAGTTCTCTT |
| Rat | |||
| Cytosolic fatty acyl thioesterase I | NM_031315 | AGTTCCTGGGTTCAATCTCC | AAGAGTGTTTACTCATGGTTCCTTT |