TABLE 2.
Effects of overexpressed fatty acid elongase on the fatty acid elongation in rat primary hepatocytes
| Recombinant Adenovirus
|
||||||||
|---|---|---|---|---|---|---|---|---|
| 14C Substrate | 14C Product | Ad-Luc | Ad-Elovl2 | Ad-Elovl5 | Ad-Elovl6 | |||
| % conversion of substrate to product | ||||||||
| 16:0 | 18:0 | 15 ± 3 | 12 ± 2 | 15 ± 3 | 51 ± 5 | |||
| 20:4,n-6 | 22:4,n-6 | 12 ± 2 | 25 ± 3 | 33 ± 5 | 12 ± 2 | |||
| 20:5,n-3 | 22:5,n-3 | 36 ± 4 | 33 ± 5 | 50 ± 5 | 33 ± 5 | |||
| 20:5,n-3 | 24:5,n-3 | <1 ± 1 | 37 ± 5 | <1 ± 1 | <1 ± 1 | |||
Rat primary hepatocytes were treated with 14C-labeled fatty acids (substrates, 100 μM; 1.6 Ci/mol) and BSA (25 μM) for 24 h. Afterward, total lipids were extracted, saponified, fractionated, and quantified by reverse-phase HPLC using a system equipped with an in-line β-scintillation counter (see supplementary Fig. I). Results are represented as percentage conversion of substrate to product, mean ± range of two or more independent assays.