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. Author manuscript; available in PMC: 2008 Jun 19.
Published in final edited form as: Br J Haematol. 2006 Jan;132(2):216–229. doi: 10.1111/j.1365-2141.2005.05850.x

Fig 1.

Fig 1

TLX1 impedes thymocyte differentiation. (A) TLX1 coding regions were subcloned upstream of the phosphoglycerate kinase promoter in the MSCV-GW retroviral vector. Abbreviations: LTR, long terminal repeat; PGK, phosphoglycerate kinase; G, GFP gene; W, woodchuck hepatitis virus posttranscriptional regulatory element. (B) Western blot analysis demonstrating TLX1 protein levels in Jurkat cells stably transduced with the MSCV-GW-TLX1 and MSCVhyg-TLX1 retroviral vectors. (C) Protocol used to evaluate effect of TLX1 expression on primary thymocyte differentiation. (D) Upper plots GFP expression in FL precursors transduced with MSCV-GW or MSCV-GW-TLX1 vectors on day 18 of liquid culture in the presence of IL-3. Lower plots GFP expression in FTOCs on day 18 of culture. Data are representative of nine thymic lobes from two independent experiments.