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. Author manuscript; available in PMC: 2008 Jun 19.
Published in final edited form as: Br J Haematol. 2006 Jan;132(2):216–229. doi: 10.1111/j.1365-2141.2005.05850.x

Fig 2.

Fig 2

TLX1 blocks thymocyte differentiation at the DN stage. (A) Time course analysis of TLX1-dependent thymocyte arrest. FL precursors transduced with either MSCV-GW (white bars) or TLX1 (black bars) were used in FTOCs. Aliquots of transduced FL precursors were maintained in parallel in liquid cultures containing IL-3. Thymocytes from organ cultures were analyzed at indicated time points for expression of GFP. Data represent the mean ± SEM for 3–4 lobes analyzed per time point. (B) Analysis of DN differentiation of FL precursors transduced with MSCV-GW (upper plots) or TLX1 (lower plots) retroviral vectors. Thymocytes from FTOCs were processed on days 3, 4 and 5 of culture and immunophenotyped for analysis of CD44/CD25 populations by gating on GFP+CD4−CD8− cells. Bivariate histograms are representative of 6–8 thymic lobes from two independent experiments.