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Inhibition of ATPase and magnesium chelatase activities by o-phenanthroline and Co(III)–ATP–o-phenanthroline. (A) o-Phenanthroline inhibition of the ATPase activities of BchH (○) and BchD + BchI (•). (B) Inhibition of magnesium chelatase activity by o-phenanthroline (•) and Co(III)–ATP–o-phenanthroline (▵). The inhibitory effect of these compounds was tested in stopped assays containing (in μg of protein): BchD, 33; BchH, 210; and BchI, 90.
