Table 2.
Assay | Subunits added and components missing | % radioactivity
|
nmol of ATP formed by phosphate transfer per min | ||
---|---|---|---|---|---|
AMP | ADP | ATP | |||
1 | BchD + BchH + BchI | 6.7 | 60.6 | 32.7 | 43.6 |
2 | BchD | 1.8 | 92.4 | 5.8 | 7.3 |
3 | BchH | 7.1 | 81.9 | 11.0 | 14.7 |
4 | BchI | 1.6 | 59.5 | 38.9 | 51.9 |
5 | BchI − ATP | 2.2 | 90.0 | 7.8 | 10.4 |
6 | BchI − Mg2+ | 1.5 | 91.5 | 7.0 | 9.3 |
Protein (μg per assay): BchD, 33; BchH, 210; BchI, 90.