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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: Matrix Biol. 2008 Jan 16;27(4):330–338. doi: 10.1016/j.matbio.2008.01.002

Figure 2. Lef1 activates the Col11a1 promoter.

Figure 2

A. This schematic depicts the Col11a1 promoter used in this study. pCol11a1(−1454)-luc encompasses bases −1454 to +269. B. C2C12 cells were transfected with pGL3 or pGL3-Col11a1(−1454)-luc, pRL-null and 200 ng of either pcDNA (−) or pCMV5B-Lef1-HA. Transcription activation is shown as fold luciferase activity over control ± SEM and corrected for transfection efficiency relative to renilla expression. C. C2C12 cells were transfected with pGL3-Col11a1(−1454)-luc, pRL-null and the indicated concentrations of pCMV5B-Lef1-HA. Data were analyzed as in B.